Diagnostics for Mycoplasma in women, men and children: how to pass and decode analysis

The success of treatment of urogenital infections more than any other field depends on the accuracy of determination of the agent. Begin diagnosis with a clinical examination, but the final decision gynecologist and venereologist or the urologist takes after studying the results of laboratory tests.

Indications for laboratory tests

Inflammatory diseases of the pelvic organs are increasingly the result of infection a persistent infection, including Mycoplasma. Doctors say the growth statistics on the incidence of:

• negonokokkovi non-specific urethritis;
• vaginosis;
• the salpingitis;
• the cervicitis;
• endometritis;
• tubal infertility.

Sluggish with mild symptoms, the disease can be caused by conditionally pathogenic Mycoplasma hominis, Ureaplasma spp. and a recognized pathogen Mycoplasma genitalium. To identify the causative agent of urogenital problems prescribed laboratory analysis of samples of biomaterials of the patient. In the course of treatment may require re-testing.

Serological test is suitable for:

• detection of Mycoplasma in acute cases of urogenital pathology in the absence of chlamydia, Trichomonas or Neisseria gonorrhoeae;
• for the diagnosis of generalized processes;
• with postnatal complications such as sepsis, bacteremia;
• infertility, miscarriage.

Special test preparation is not required. The blood analysis carried out in the morning on an empty stomach. The answer is ready in 1.5 hours after the start of the study.

As evidenced by the results

Bacteriological study borderline of norm and pathology is the concentration (titer) of Mycoplasma and Ureaplasma 10⁴ CFU/ml. Fewer prokaryotes indicates health, and more can cause anxiety. Detection of clinically significant titles in the course of analysis becomes the reason for further tests on the resistance of pathogen to antibiotics. The patient then receives recommendations for treatment of infection.

In studies by PCR on the form write "negative" if the DNA of Mycoplasma is not detected, or "positive", if any. In the second case it is possible the carrier or active infectious process.

Qualitative differences in the detection of mycoplasmal DNA can only give a real-time PCR. Then the form will contain the titles of the infection.

Immunological studies on antibodies of class IgM and IgG to Mycoplasma issue a response in the form of "negative/doubtful/positive". The first option can be deciphered as:

• infection occurred in up to 2 weeks;
• the infection did not cause a strong immuneanswer;
• no sign of infection.

The technician will put the answer in cases where the sample will find less than 8 less IgM and 5 IgG antibodies.

Doubt will cause samples with the presence of 9 IgM and 5 IgG antibodies.

Titers of positive responses are as follows:

Caption	anti-Myc. hominis IgM	anti-Myc. hominis IgG
weakly positive	10 - 30	10
positive	40 - 1100	20
selenoproteins	> 1100	≥40

These figures indicate that the germ-carriers or current infection.

Possible error

Independent interpretation of results one of even the most accurate test will not be useful. Information should be analysed comprehensively taking into account the findings of the clinical examination and the disease.

Tests can give false answers. Errors occur under the following conditions:

• violations of the rules of sampling;
• analyses on the background of antibacterial therapy;
• sample contamination by extraneous DNA;
• inhibition of PCR by hemoglobin or heparin.

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